Journal
CHEMOSENSORS
Volume 10, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/chemosensors10120521
Keywords
droplet microfluidics; single cells; cell signaling profiling; high spatiotemporal resolution
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This study developed a microfluidic platform based on surface energy well (SEW) to achieve high spatiotemporal-resolved signal profiling at the single-cell level. The platform allows for controlled droplet replacement, reliable single-cell patterning, and successive treatment of adherent cells with reagent droplets.
A small indentation embedded in a microchannel creates a surface energy well (SEW) for a confined droplet due to surface energy release. Inspired by this, we developed a SEW-based microfluidic platform to realize high spatiotemporal-resolved signal profiling at the single-cell level applying droplet stimulus on a single chip. The method allows for controlled droplet replacement within only 3 s with almost 100% exchange efficiency, reliable single-cell patterning of adherent cells and successive treatment of adherent cells with reagent droplets. Furthermore, the PDGFR/Akt pathway served as a model system for evaluating the performance of the SEW-based method in determining the effects of ligand stimulation duration (3 s to 3 min) on receptor phosphorylation. The novel strategy offers a general platform for probing the temporal dynamics of single cells, as well for monitoring rapid chemical reactions in various applications.
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