Journal
BIOSENSORS-BASEL
Volume 13, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/bios13020210
Keywords
Fe3O4@Au; lateral flow assay; respiratory bacteria; SERS; 4-mercaptophenylboronic acid
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In this study, a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip was designed for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. The use of surface enhanced Raman scattering (SERS) tags improved the sensitivity and specificity, making it suitable for the detection of respiratory bacteria samples.
The rapid diagnosis and detection of respiratory bacteria at the early stage can effectively control the epidemic spread and bacterial infection. Here, we designed a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. In this assay, the surface enhanced Raman scattering (SERS) tags were designed through combining magnetite Raman enhancement nanoparticle Fe3O4@Au/DTNB and recognition element 4-mercaptophenylboronic acid (4-MPBA). Further, 4-MPBA could capture multiple bacteria in a complex environmental solution. Based on the strategies, Fe3O4@Au/DTNB-mediated magnetic enrichment and 4-MPBA-mediated universal capture capabilities improved the detection sensitivity, the limits of detection for S. aureus and S. pneumoniae were as low as 8 and 13 CFU mL(-1), respectively, which were more sensitive than those of colloidal gold method. The Fe3O4@Au/DTNB/Au/4-MPBA-LFA also exhibited good reproducibility, excellent specificity, and high recovery rates in sputum samples, indicating its potential application in the detection of respiratory bacteria samples.
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