Related references
Note: Only part of the references are listed.
Article
Chemistry, Analytical
Xinyi Luo et al.
Summary: Early and accurate diagnosis of viruses is critical for control of the pandemic. In this study, a new gene RNA detection platform was developed for quantitative detection of viral RNA with high sensitivity and specificity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Tongjia Kang et al.
Summary: Achieving superhigh sensitivity is the ultimate goal in modern bio-detection. Nucleic acid amplification technologies (NAATs) are revolutionizing the field, offering novel methods for diagnosis with high efficiency and cost-effectiveness. This review discusses the principles, recent advancements, strengths, and challenges of different NAATs, with a focus on rapid and accurate point-of-care diagnosis. The application of these technologies in the context of the COVID-19 pandemic is also explored, emphasizing the significance of NAATs in combating the disease. Furthermore, the potential for enhancing sensitivity and specificity in biodetection through NAATs is discussed, aiming to fully exploit the capabilities of each technique.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Ziyue Li et al.
Summary: In this study, a simple, sensitive, and instrument-free diagnostic method for SARS-CoV-2 was developed using a self-contained microfluidic system. The system integrates amplification, CRISPR cleavage, and lateral flow detection, allowing contamination-free visual detection. The method showed excellent sensitivity, specificity, and accuracy when clinically validated with COVID-19 samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medicine, General & Internal
Maya Zahavi et al.
Summary: The Lucira COVID-19 Test kit demonstrates high sensitivity and specificity in identifying the virus, particularly accurate for symptomatic participants. Additionally, a significant positive correlation is found between Ct values and the time-to-positivity with Lucira.
Article
Chemistry, Analytical
Juan Garcia-Bernalt Diego et al.
Summary: Nucleic acid amplification diagnostics, although sensitive and specific, lack speed, robustness, affordability, and user-friendliness. This study combines loop-mediated isothermal amplification technology with a handheld portable device (SMART-LAMP) to enable real-time nucleic acid amplification reactions through simple colorimetric measurements. The device is controlled by a dedicated smartphone app and can be used for diagnosing different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19. The combination of long-term stabilized LAMP master mixes and the SMART-LAMP device improves point-of-care diagnosis of infectious diseases in low-resource settings, and can be easily adapted for diagnosing other infectious diseases.
Article
Chemistry, Analytical
Hanh An Nguyen et al.
Summary: In this study, a rotatable paper device integrating the loop-mediated isothermal amplification (RT-LAMP) technique and naked-eye readout was developed for the detection of infectious pathogens. The device utilized hydroxyl radicals to decolorize a food additive called carmoisine, and the color change of the solution indicated the presence of target DNA. The rotatable paper device successfully detected SARS-CoV-2, SARS-CoV, and Enterococcus faecium in milk within 70 minutes using only visual observation. The device offers a portable and low-cost tool for pathogen detection in resource-limited environments.
Article
Biochemical Research Methods
Yingying Xue et al.
Summary: Digital loop-mediated isothermal amplification (dLAMP) is a popular method for nucleic acid detection due to its advantages, but suffers from inaccurate quantification. A prehybridization-induced enhancement (PIE) strategy has been developed to improve digital efficiency without the need for new primers or additional operations, providing new insights into the reaction dynamics of dLAMP. The PIE strategy could be applied to other digital amplification methods for enhanced efficiency.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Infectious Diseases
Leslie J. Donato et al.
Summary: The Cue Health POC nucleic acid amplification test demonstrated very good positive and negative percent agreement with central laboratory tests, making it useful in settings where accurate POC testing is needed to facilitate management of COVID-19 suspected patients.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Chemistry, Analytical
Jiasi Wang et al.
Summary: Cervical cancer is a leading cause of cancer deaths among women in developing countries, highlighting the need for a rapid and low-cost HPV detection method for screening. The dLAMP assay and self-digitization chip demonstrated reliable genotyping and quantitative detection of 14 high-risk HPVs, showing potential for simple, rapid, and cost-effective diagnosis of HPV infection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yaqin Zhang et al.
Summary: The development of a gold nanoparticle-based visual assay combined with CRISPR/Cas12a-assisted RT-LAMP for rapid and sensitive detection of SARS-CoV-2 shows promise in improving COVID-19 screening efforts. This method is easy to operate and has high-throughput testing capability, with potential applications in screening suspected individuals in various settings.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shijun Li et al.
Summary: CRISPR-top is a novel diagnostic method that integrates target pre-amplification and CRISPR-mediated detection in one reaction mixture for SARS-CoV-2 detection. This simple, rapid, accurate, and highly sensitive method can return results within 60 minutes, making it suitable for clinical and resource-challenged settings.
Article
Multidisciplinary Sciences
Thanyarat Chaibun et al.
Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Rui Jie Li et al.
Summary: The EPCM generates heat when in contact with water, allowing it to maintain a constant incubation temperature for enzymatic amplification. This enables a low-cost, disposable, electricity-free diagnostic test, demonstrated in detecting SARS-Cov-2 in saliva samples without instrumentation or using a palm-size CCD camera for real-time amplification monitoring.
Article
Chemistry, Analytical
Kawin Nawattanapaiboon et al.
Summary: This study developed a visual diagnostic platform for SARS-CoV-2 based on colorimetric RT-LAMP with sensitivity and specificity comparable to commercial qRT-PCR assays. The method showed high accuracy and reliability in clinical specimens, indicating its potential beneficial use as a diagnostic platform for COVID-19 screening.
Review
Infectious Diseases
Qingmei Han et al.
JOURNAL OF INFECTION
(2020)
Article
Multidisciplinary Sciences
Peng Zhou et al.
Review
Biophysics
Neeraja Ravi et al.
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Virology
Lena Mautner et al.
Review
Biotechnology & Applied Microbiology
Dorian Thompson et al.
SENSORS AND ACTUATORS REPORTS
(2020)
Article
Biochemical Research Methods
Jason E. Kreutz et al.
Article
Biochemical Research Methods
Jiasi Wang et al.
Article
Chemistry, Multidisciplinary
Jesus Rodriguez-Manzano et al.
Article
Biochemical Research Methods
Motoki Goto et al.
Article
Biochemistry & Molecular Biology
Tsugunori Notomi et al.
NUCLEIC ACIDS RESEARCH
(2000)
