Journal
BIOSENSORS-BASEL
Volume 13, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/bios13010021
Keywords
SERS probe; cyano Raman Tag; pH responsive; environment detection
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As a crucial parameter in biochemical analysis, the pH value plays an essential role in cell function, food preservation and production, as well as soil and water sources. Therefore, it is increasingly important to explore pH detection methods. This paper introduces a pH-responsive SERS probe using a cyano Raman tag, which can detect pH values based on the displacement of the cyano Raman peak. The cyano Raman tag exhibits excellent sensitivity within the pH range of 3.0-9.0, with a limit of detection of pH 0.33, and shows anti-interference performance and stability (the relative standard deviation is calculated to be 6.68%, n = 5). These results suggest that this pH SERS probe with the Raman cyano tag could open up new research directions in biological detection, bioimaging, and environmental detection.
As one of the most important parameters of biochemical analysis and detection, the pH value plays a very important role in cell function, food preservation and production, soil and water sources, and other applications. This makes it increasingly important to explore pH detection methods in depth. In this paper, a pH-responsive SERS probe based on the cyano Raman Tag was designed to realize pH sensing detection through the influence of the pH value of analytes on the displacement of the cyano Raman peak in the SERS probe. This cyano Raman tag exhibited not only excellent sensitivity in the liner range of pH 3.0-9.0 with a limit of detection (LOD) of pH 0.33, but also the anti-interference performance and stability (the relative standard deviation (RSD) was calculated to be 6.68%, n = 5). These results indicated that this pH SERS probe with the Raman cyano tag can provide new research ideas for future biological detection, bioimaging, and environmental detection.
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