Journal
BIOSENSORS-BASEL
Volume 13, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/bios13020164
Keywords
colorimetric assay; gold nanoparticles; 4-Mercaptophenyl boronic acid; polyethylene glycol 400; repulsive the interparticle crosslinking; sialic acid
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A novel colorimetric method for the determination of sialic acid (SA) was developed using 4-mercaptophenyl boronic acid (4-MPBA) as the analyte's recognition molecule, and negative charge PEG (400) was used to repulsive the interparticle crosslinking. The proposed sensing platform showed a linear relationship between the ratio of the absorbance intensity (A(525)/A(660)) and concentration of SA from 0.05 to 8 mM (R-2 = 0.997), with a detection limit of 48 mu M. This gold-based colorimetric sensor is easy to fabricate, reproducible in its test performance, and has been successfully applied for the detection of SA in biological and healthcare product samples.
Sialic acid (SA) is a well-known component of glycoproteins, which have applications in various functional processes on the cell's surface. The colorimetric is a simpler and more convenient method for measuring SA due to its low-cost apparatus and visual signal changes. This work focused on the unpredictable interparticle crosslinking aggregation of the functionalized gold nanoparticles (AuNPs) in complex media. We proposed a balance of the Derjaguin-Landau-Verwey-Overbeek (DLVO)-type aggregation and molecule-based interaction method to solve this problem. Here, we report a novel colorimetric assay for the determination of SA using 4-mercaptophenyl boronic acid (4-MPBA) as an analyte's recognition molecule, and negative charge PEG(400) was used to repulsive the interparticle crosslinking. The proposed sensing platform shows a linear relationship between the ratio of the absorbance intensity (A(525)/A(660)) and concentration of SA from 0.05 to 8 mM (R-2 = 0.997) and a detection limit of 48 mu M was observed. The novel gold-based colorimetric sensor is easy to fabricate, reproducible in its test performance and has been successfully applied for the detection of SA in biological and healthcare product samples.
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