Journal
BIOSENSORS-BASEL
Volume 12, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/bios12121161
Keywords
immunosensor; SARS-CoV-2; N protein
Funding
- Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [45141.UNI767.19114. 15072019]
- [8888.452396/20019-01]
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This study developed a biosensor-based ELISA immunoassay for monitoring SARS-CoV-2 antibodies in human serum samples. It employed a specific receptor and a secondary antibody to detect and quantify the antibody concentration using the chronoamperometric method.
The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3 ',5,5 '-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 1:1000-1:200 v/v in phosphate buffer solution (PBS), and the detection limit calculated was 1:4947 v/v. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction.
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