Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au-2D WS2 Nano-Hybrid Materials

In this study, we proposed a novel Ru(bpy)(3)(2+)-Au-WS2 nanocomposite (Ru-Au-WS2 NCs) nano-hybrid electrochemiluminescence (ECL) probe for the highly sensitive detection of carcinoembryonic antigen (CEA). This system utilizes Au nanoparticles (Au NPs) as a bridge to graft the high-performance of a Ru(bpy)(3)(2+) ECL emitter and WS2 nanosheet with excellent electrochemical performance into an ECL platform, which shows outstanding anodic ECL performance and biosensing platform due to the synergetic effect and biocompatibility of Au NPs and WS2 nanosheet. Because the ECL intensity of Ru(bpy)(3)(2+) is sensitively affected by the antibody-antigen insulator, a preferable linear dependence was obtained in the concentration range of CEA from 1 pg center dot mL(-1) to 350 ng center dot mL(-1) with high selectivity (LOD of 0.3 pg center dot mL(-1), S/N = 3). Moreover, the ECL platform had good reproducibility and stability and exhibited excellent anti-interference performance in the detection process of CEA. We believe that the platform we have developed can expand the opportunities for the detection of additional high specificity-related antibodies/antigens and demonstrate broad prospects for disease diagnosis and biochemical research.

Automated summary

In this study, a novel Ru(bpy)(3)(2+)-Au-WS2 nanocomposite ECL probe was developed for the detection of carcinoembryonic antigen (CEA). The probe showed high sensitivity and selectivity due to the synergistic effect and biocompatibility of Au nanoparticles (Au NPs) and WS2 nanosheet. The ECL platform exhibited excellent anodic ECL performance, good reproducibility, stability, and anti-interference performance.