Microbial Removal of Petroleum Hydrocarbons from Contaminated Soil under Arsenic Stress

The contamination of soils with petroleum and its derivatives is a longstanding, widespread, and worsening environmental issue. However, efforts to remediate petroleum hydrocarbon-polluted soils often neglect or overlook the interference of heavy metals that often co-contaminate these soils and occur in petroleum itself. Here, we identified Acinetobacter baumannii strain JYZ-03 according to its Gram staining, oxidase reaction, biochemical tests, and FAME and 16S rDNA gene sequence analyses and determined that it has the ability to degrade petroleum hydrocarbons. It was isolated from soil contaminated by both heavy metals and petroleum hydrocarbons. Strain JYZ-03 utilized diesel oil, long-chain n-alkanes, branched alkanes, and polycyclic aromatic hydrocarbons (PAHs) as its sole carbon sources. It degraded 93.29% of the diesel oil burden in 7 days. It also had a high tolerance to heavy metal stress caused by arsenic (As). Its petroleum hydrocarbon degradation efficiency remained constant over the 0-300 mg/L As(V) range. Its optimal growth conditions were pH 7.0 and 25-30 degrees C, respectively, and its growth was not inhibited even by 3.0% (w/v) NaCl. Strain JYZ-03 effectively bioremediates petroleum hydrocarbon-contaminated soil in the presence of As stress. Therefore, strain JYZ-03 may be of high value in petroleum- and heavy-metal-contaminated site bioremediation.

Automated summary

The contamination of soils with petroleum and its derivatives is a persistent and growing environmental problem, however the interference of heavy metals co-contaminating these soils is often overlooked. Acinetobacter baumannii strain JYZ-03 was identified as capable of degrading petroleum hydrocarbons and isolated from soil contaminated by both heavy metals and petroleum hydrocarbons. Strain JYZ-03 effectively bioremediates petroleum hydrocarbon-contaminated soil in the presence of heavy metal stress, making it valuable in the bioremediation of petroleum- and heavy-metal-contaminated sites.