Journal
FRONTIERS IN NUTRITION
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.1077893
Keywords
fluorescent aptasensor; sulfadimethoxine; oxytetracycline; silica nanoparticles; quantitative detection
Categories
Funding
- National Natural Science Foundation of China [31671939]
- Postgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission [CYS21273]
- Chongqing of University Innovation Research Group Project [CXQT20031]
- Chongqing Natural Science Foundation [cstc2021jcyj-msmX0314]
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In this study, a dual fluorescent aptasensor based on mesoporous silica was developed for simultaneous detection of sulfadimethoxine and oxytetracycline in animal-derived foods. The aptasensor showed good detection performance and recovery rates. This study offers a new possibility for analyzing other antibiotics, biotoxins, and biomolecules.
Herein, we developed a dual fluorescent aptasensor based on mesoporous silica to simultaneously detect sulfadimethoxine (SDM) and oxytetracycline (OTC) in animal-derived foods. We immobilized two types of aptamers modified with FAM and CY5 on the silica surface by base complementary pairing reaction with the cDNA modified with a carboxyl group and finally formed the aptasensor detection platform. Under optimal conditions, the detection range of the aptasensor for SDM and OTC was 3-150 ng/mL (R-2 = 0.9831) and 5-220 ng/mL (R-2 = 0.9884), respectively. The limits of detection for SDM and OTC were 2.2 and 1.23 ng/mL, respectively. The limits of quantification for SDM and OTC were 7.3 and 4.1 ng/mL, respectively. Additionally, the aptasensor was used to analyze spiked samples. The average recovery rates ranged from 91.75 to 114.65% for SDM and 89.66 to 108.94% for OTC, and all coefficients of variation were below 15%. Finally, the performance and practicability of our aptasensor were confirmed by HPLC, demonstrating good consistency. In summary, this study was the first to use the mesoporous silica-mediated fluorescence aptasensor for simultaneous detection of SDM and OTC, offering a new possibility to analyze other antibiotics, biotoxins, and biomolecules.
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