Journal
CELL RESEARCH
Volume 26, Issue 2, Pages 239-253Publisher
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2015.152
Keywords
NudC; actin dynamics; ciliogenesis; cofilin 1; zebrafish development
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Funding
- Ministry of Science and Technology of China [2012CB945004, 2013CB945603, 2011CBA01001]
- National Natural Science Foundation of China [31125017, 31190063, 31100975, 31571446, 31471259]
- Ministry of Education of China [20110101110103]
- Natural Scientific Foundation of Zhejiang Province, China [LY14C070001, LY14C070002]
- 111 Project [B13026]
- Department of Science and Technology of Zhejiang Province [2012C37089, 2013TD13]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents
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Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates actin cytoskeleton and ciliogenesis by stabilizing cofilin 1.
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