Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 13, Pages 2466-2473Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600247
Keywords
High-performance liquid chromatography; Microwave-assisted extraction; Phytosterols; Solid-phase extraction; Tobacco
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Funding
- National Natural Science Foundation of China [21275088]
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Acid hydrolysis and alkaline saponification were incorporated into a microwave-assisted extraction process for the simultaneous extraction of free and conjugated phytosterols from tobacco. The crude extract of the microwave-assisted extraction was purified by C-18 solid-phase extraction and then determined by high-performance liquid chromatography. Phytosterols of cholesterol, ergosterol, stigmasterol, campesterol, and beta-sitosterol were determined by chromatographic quantification. The multiple parameters of microwave-assisted extraction were optimized by a uniform design method. The optimal ratio of extraction ethanol solvent to tobacco mass was 30 mL/g. The microwave-assisted extraction acid hydrolysis was carried out in sulfuric acid medium by heating for 10min at 55 degrees C. The microwave-assisted extraction alkaline saponification was performed after adding excessive sodium hydroxide by heating another 10min. The repeatability of the proposed method was acceptable with recoveries from 69.68 to 88.17% for the phytosterols. Five target phytosterols were all found in the tobacco samples, and the contents were significantly different in samples from different producing areas.
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