Journal
COMMUNICATIONS BIOLOGY
Volume 5, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s42003-022-04269-w
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Funding
- Inserm
- Sorbonne Universite
- European Research Council (ERC) [AIG-250349]
- Biology for Psychiatry Laboratory of Excellence (Labex Bio-Psy, Investissements d'Avenir) [ANR-11-IDEX-0004-02]
- Fondation pour la Recherche Medicale (FRM) [DPA20140629798, FDT201805005390]
- ANR Epitraces [ANR-16-CE16-0018]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE16-0018] Funding Source: Agence Nationale de la Recherche (ANR)
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Neuronal DNA modifications, including methylation outside CpG context and abundant 5-hydroxymethylation, show distinct patterns in striatal projection neurons expressing D1 or D2 dopamine receptors. DNA methylation differences mainly occur in large genomic clusters related to differentially expressed genes, while hydroxymethylation differences are scattered and affect transcription factor binding sites.
Neuronal DNA modifications differ from those in other cells, including methylation outside CpG context and abundant 5-hydroxymethylation whose relevance for neuronal identities are unclear. Striatal projection neurons expressing D1 or D2 dopamine receptors allow addressing this question, as they share many characteristics but differ in their gene expression profiles, connections, and functional roles. We compare translating mRNAs and DNA modifications in these two populations. DNA methylation differences occur predominantly in large genomic clusters including differentially expressed genes, potentially important for D1 and D2 neurons. Decreased gene body methylation is associated with higher gene expression. Hydroxymethylation differences are more scattered and affect transcription factor binding sites, which can influence gene expression. We also find a strong genome-wide hydroxymethylation asymmetry between the two DNA strands, particularly pronounced at expressed genes and retrotransposons. These results identify novel properties of neuronal DNA modifications and unveil epigenetic characteristics of striatal projection neurons heterogeneity.
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