Open questions on liquid-liquid phase separation

Liquid-liquid phase separation (LLPS) underlies the formation of intracellular membraneless compartments in biology and may have played a role in the formation of protocells that concentrate key chemicals during the origins of life. While LLPS of simple systems, such as oil and water, is well understood, many aspects of LLPS in complex, out-of-equilibrium molecular systems remain elusive. Here, the author discusses open questions and recent insights related to the formation, function and fate of such condensates both in cell biology and protocell research. Liquid-liquid phase separation (LLPS) underlies the formation of intracellular membraneless compartments in biology and may have played a role in the formation of protocells that concentrate key chemicals during the origins of life. While LLPS of simple systems, such as oil and water, is well understood, many aspects of LLPS in complex, out-of-equilibrium molecular systems remain elusive. Here, the author discusses open questions and recent insights related to the formation, function and fate of such condensates both in cell biology and protocell research.

Automated summary

Liquid-liquid phase separation (LLPS) is essential for the formation of intracellular membraneless compartments and may have played a role in the origins of protocells. While LLPS in simple systems is well understood, there are still many unknowns about LLPS in complex molecular systems. In this article, the author discusses the formation, function, and fate of LLPS condensates in both cell biology and protocell research.