Journal
PHARMACEUTICALS
Volume 15, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/ph15111382
Keywords
baculovirus; encapsulation; wound healing; angiogenesis; gene delivery; VEGFA; revascularization; wound dressing; targeted delivery; alginate; chitosan
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Funding
- Canadian Institute of Health Research (CIHR) [252743]
- Canada Graduate Scholarship-Doctoral Award from Natural Sciences and Engineering Research Council (NSERC) [569661-2022]
- Islamic Development Bank Scholarship [2020-245622]
- Ministry of Higher Education of the Arab Republic of Egypt
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This study demonstrates that encapsulating a recombinant baculovirus expressing VEGFA in chitosan-coated alginate hydrogels enhances cell migration, angiogenesis, and wound healing, while also providing antimicrobial properties and sustained delivery of the virus.
Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization. Here, a biosafe recombinant baculovirus expressing VEGFA tagged with EGFP is encapsulated in chitosan-coated alginate hydrogels using ionic cross-linking. The VEGFA, delivered by the baculovirus, significantly improves cell migration and angiogenesis to assist with the wound-healing process and revascularization. Moreover, the hydrogels have an encapsulation efficiency of 99.9%, no cytotoxicity, antimicrobial properties, good blood compatibility, promote hemostasis, and enable sustained delivery of baculoviruses over eight days. These hydrogels sustain baculovirus delivery and may have clinical implications in wound dressings or future gene therapy applications.
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