SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma

SLC17A9 is a vesicular ATP transport protein that plays an important role in determining cell functions and the onset and progression of different diseases. In this study, SLC17A9 was initially identified as a potential diagnostic and prog-nostic risk biomarker for clear cell renal cell carcinoma (ccRCC). Then, the aberrant expression levels of SLC17A9 were confirmed in both the cell lines and clinical tissues. Mechanistically, SLC17A9 could upregulate the expression of PTHLH, thus promoting epithelial-mesenchymal transition (EMT) in ccRCC. Functionally, SLC17A9 knockdown inhibited the proliferation, migration, and invasion activity of renal cancer cells, whereas its overexpression led to stronger cell viability and more malignant phenotype in vitro. The overexpression of SLC17A9 in vivo could significantly contribute to the growth of tumors. Finally, we found that SLC17A9 might be related to the drug resistance of vorinostat. Cumulatively, this study demonstrated that the SLC17A9-PTHLH-EMT axis could promote the progres-sion of ccRCC.

Automated summary

SLC17A9, a vesicular ATP transport protein, has been identified as a potential diagnostic and prognostic risk biomarker for clear cell renal cell carcinoma (ccRCC). Aberrant expression levels of SLC17A9 were confirmed in both cell lines and clinical tissues. Mechanistically, SLC17A9 could upregulate the expression of PTHLH, promoting epithelial-mesenchymal transition (EMT) in ccRCC. Functionally, SLC17A9 knockdown inhibited the proliferation, migration, and invasion of renal cancer cells, while overexpression led to increased cell viability and a more malignant phenotype in vitro. The overexpression of SLC17A9 in vivo significantly contributed to tumor growth. Additionally, SLC17A9 may be associated with vorinostat drug resistance.