Journal
BIOMEDICINES
Volume 11, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines11020430
Keywords
sorting; amniotic fluid stem cells; transcriptome; stemness
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Human amniotic fluid cells (hAFSCs) are a heterogeneous population with stem cell characteristics. This study compares the gene expression profiles of adherent amniotic fluid cells and their c-Kit(+) subpopulation through single-cell and bulk RNAseq analysis. Differences in cellular distribution, pathways related to stemness, and secretome composition were identified between the two populations. Selecting c-Kit positive fractions with higher potential could be important in regenerative medicine applications.
Human amniotic fluid cells (hAFSCs) are a fascinating foetal cell-type that have important stem cell characteristics; however, they are a heterogeneous population that ranges from totally differentiated or progenitor cells to highly multipotent stem cells. There is no single approach to isolating the stem cell component, but the selection of a subpopulation of hAFSCs expressing c-Kit is widely employed, while a deep characterization of the two populations is still lacking. Here we performed single-cell and bulk RNAseq analysis to compare the gene expression profiles of adherent amniotic fluid cells and their subpopulation c-Kit(+). Information deriving from this high throughput technology on the transcriptome was then confirmed for specific targets with protein expression experiments and functional analysis. In particular, transcriptome profiling identified changes in cellular distribution among the different clusters that correlated with significant differential expression in pathways related to stemness, proliferation, and cell cycle checkpoints. These differences were validated by RT-PCR, immunofluorescence, WB, and cell cycle assays. Interestingly, the two populations produced secretomes with different immune-modulating and pro-regenerative potentials. Indeed, the presence of TGF beta, HGF, IDO was higher in EVs deriving from c-Kit(+) cells, unlike IL-6. These results suggest the existence of deep intra-population differences that can influence the stemness profile of hAFSCs. This study represents a proof-of-concept of the importance of selecting c-Kit positive fractions with higher potential in regenerative medicine applications.
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