Can Peat Amendment of Mars Regolith Simulant Allow Soybean Cultivation in Mars Bioregenerative Life Support Systems?

Higher plants will play a key role in human survival in Space, being able to regenerate resources and produce fresh food. However, the creation of a fertile substrate based on extra-terrestrial soils is still a challenge for space cultivation. We evaluated the adaptability of soybean (Glycine max (L.) Merr.) cultivar 'Pr91M10 ' to three substrates, the Mojave Mars regolith Simulant MMS-1, alone (R100), and in a mixture with blond sphagnum peat at two different volumes, 85:15 (R85P15) and 70:30 (R70P30), in plants directly sown on the substrates or transplanted after sowing on peat. The low pH of peat (4.34) allowed the mitigation of the alkalinity of the Mars regolith simulant (pH 8.86), lowering the initial pH to neutral (6.98, R85P15), or subacid to neutral (6.33, R70P30) values. Seed germination reached the highest percentage in the shortest time in the mixture of regolith simulant with 15% of peat. The cultivation substrate did not affect the plant growth and nutritional status. However, a significant interaction between the substrate and planting method was found in several growth parameters, with the highest positive effects observed in plants resulting from direct sowing on the regolith mixture with peat.

Automated summary

Higher plants are important for human survival in Space as they can regenerate resources and produce fresh food. However, developing a fertile substrate using extra-terrestrial soils remains a challenge. In this study, we evaluated the adaptability of soybean cultivar 'Pr91M10' to three substrates, including a Mars regolith simulant alone and mixed with blond sphagnum peat. The results showed that the addition of peat improved the germination rate and had a positive effect on plant growth when directly sown on the regolith mixture.