Journal
PLANTS-BASEL
Volume 12, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/plants12020332
Keywords
acetolactate synthase resistance; smooth pigweed; point mutations; alleles; single-seed DNA extraction; resistance management
Categories
Ask authors/readers for more resources
Poor control of Amaranthus spp. with herbicides inhibiting acetolactate synthase (ALS) has been observed in soybean fields in north-eastern Italy. Extensive sampling confirmed the presence of ALS-resistant populations of Amaranthus hybridus and identified mutant ALS alleles responsible for resistance. Pre-emergence herbicides metribuzin, clomazone, and metobromuron can be used as alternative management strategies to limit resistance spread.
Poor control of Amaranthus spp. with herbicides inhibiting acetolactate synthase (ALS) has been observed for several years in soybean fields in north-eastern Italy, but to date only a few ALS-resistant populations have been confirmed. An extensive sampling of putatively resistant Amaranthus accessions was completed in the Friuli Venezia Giulia region, across an arable land area of about 3000 km(2). In total, 58 accessions were tested to confirm their resistance status, recognize the Amaranthus species, identify the mutant ALS alleles endowing the resistance and determine the efficacy of 3 pre-emergence herbicides. Most accessions resulted in cross-resistance to thifensulfuron-methyl and imazamox. Genomic DNA were extracted from single seeds with a newly developed protocol; an allele-specific PCR assay revealed the presence of the 574-leucine in 20 accessions, of the 574-methionine in 22, and of both alleles in 9 accessions. The two variants showed a different spatial distribution. All resistant populations were ascribed to A. hybridus. A. hybridus resistant to ALS herbicides is well-established in this Italian region and its resistance is due to two ALS mutant alleles. Metribuzin, clomazone and metobromuron can be used as alternative herbicides to be applied in pre-emergence and they should be integrated into the management strategies to limit the spread of resistance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available