Journal
PLANTS-BASEL
Volume 11, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/plants11212984
Keywords
G4 Eurasian avian-like H1N1 virus; neuraminidase; vaccine; molecular farming; immune response
Categories
Funding
- Next-Generation BioGreen 21 Program (System and Synthetic Agrobiotech Center), Rural Development Administration [PJ01335801]
- National Research Foundation, Ministry of Science, Technology, and Future Planning, Korea [NRF-2020R1A2C4002294]
- Korean government (MSIT) [NRF-2017M3A9G6068245, NRF-2021R1C1C1012962]
Ask authors/readers for more resources
Influenza viruses, especially G4 EA H1N1, pose a major public health threat. In this study, G4NA derived from plants was shown to induce significant humoral immune responses and antigen-specific CD4 T cell activation, making it a potential vaccine candidate against G4 EA H1N1.
Influenza viruses are a major public health threat that causes repetitive outbreaks. In recent years, genotype 4 (G4) reassortant Eurasian avian-like (EA) H1N1 (G4 EA H1N1) has garnered attention as a potential novel pandemic strain. The necessity of developing vaccines against G4 EA H1N1 is growing because of the increasing cases of human infection and the low cross-reactivity of the strain with current immunity. In this study, we produced a G4 EA H1N1-derived neuraminidase (G4NA) as a vaccine candidate in Nicotiana benthamiana. The expressed G4NA was designed to be accumulated in the endoplasmic reticulum (ER). The M-domain of the human receptor-type tyrosine-protein phosphatase C was incorporated into the expression cassette to enhance the translation of G4NA. In addition, the family 3 cellulose-binding module and Brachypodium distachyon small ubiquitin-like modifier sequences were used to enable the cost-effective purification and removal of unnecessary domains after purification, respectively. The G4NA produced in plants displayed high solubility and assembled as a tetramer, which is required for the efficacy of an NA-based vaccine. In a mouse immunization model, the G4NA produced in plants could induce significant humoral immune responses. The plant-produced G4NA also stimulated antigen-specific CD4 T cell activation. These G4NA vaccine-induced immune responses were intensified by the administration of the antigen with a vaccine adjuvant. These results suggest that G4NA produced in plants has great potential as a vaccine candidate against G4 EA H1N1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available