Morphoanatomical, Physiological, and Biochemical Indicators in Lactuca sativa L. Germination and Growth in Response to Fluoride

Fluoride is one of the main phytotoxic environmental pollutants, and high concentrations (10-30 mg L-1) are commonly detected in surface and groundwater. Little, however, is known about the effects of this pollutant on crops that require irrigation during their development, which, in addition to phytotoxicity, may cause negative human health effects. Thus, the aim of this study was to characterize the effects of potassium fluoride (KF) on the germination of lettuce seeds and identify the physiological and anatomical markers of this pollutant's action on plants exposed to it during growth. Initially, lettuce seeds were sown in gerboxes and soaked in solutions containing 0 mg L-1, 10 mg L-1, 20 mg L-1, and 30 mg L-1 KF. Plants grown in a greenhouse were treated daily with KF irrigation at the same KF concentrations for 40 days. KF exposure reduced the germination rate and germination speed index of lettuce seeds at 20 mg L-1 and 30 mg L-1, resulting in compromised root development at the highest KF concentration. Lettuce plants displayed a slight photosynthesis reduction and a significant photochemical efficiency decrease after exposures to all KF concentrations. Lower chlorophyll contents and nitrogen balance indices were observed in plants exposed to 30 mg L-1 KF. On the other hand, increases in phenolic compounds and malondialdehyde were noted with increasing KF concentrations. Lettuce plants can, therefore, accumulate fluoride in leaves when irrigated with KF-rich water. The investigated physiological and biochemical variables were proven to be adequate fluoride action biomarkers in lettuce plants and may become an important tool in the study of olericulture contaminants.

Automated summary

This study examined the effects of potassium fluoride (KF) on the germination of lettuce seeds and the subsequent growth of the plants. Results showed that exposure to high concentrations of KF reduced germination rate and compromised root development. Lettuce plants also exhibited reduced photosynthesis and photochemical efficiency, as well as lower chlorophyll contents and nitrogen balance indices. On the other hand, there were increases in phenolic compounds and malondialdehyde with higher KF concentrations. These findings suggest that lettuce plants can accumulate fluoride when irrigated with KF-rich water, and the investigated physiological and biochemical markers can be useful in studying contaminants in horticulture.