Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction

This study aims to compare the suitability of three extraction techniques (cold pressing, Soxhlet and supercritical fluid extraction (SFE)) to isolate oil from cherry seeds. Oils were examined in terms of extraction yield, fatty acids profile, tocopherols yield and antioxidant activity. Additionally, influence of SFE parameters was evaluated using one-factor-at-a-time design with pressure (200-350 bar), temperature (40-70 degrees C), flow rate (0.2-0.4 kg/h) and particle size (800 mu m). Oil yields ranged from 2.50% to 13.02%, whereas the highest yield was achieved with SFE. Samples were rich in polyunsaturated fatty acids, regardless of the applied extraction technique. The main fatty acids were linoleic (46.32-47.29%), oleic (40.89-41.65%), palmitic (6.56-8.00%) and stearic (2.21-2.30%) acid. Total tocopherols yield was between 16.63 mg/100 g oil and 60.61 mg/100 g oil, and highest yield was achieved with SFE. Among the tocopherols, gamma-tocopherol was the most abundant, followed by alpha-, delta- and beta-tocopherol. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) cation (ABTS) assays, and the results indicated that SFE extracts exhibited better or comparable antioxidant potential compared to traditional techniques. The comparison between modern and conventional extractions for oil recovery demonstrates pros and cons for the possibility of industrial application.

Automated summary

The suitability of three extraction techniques (cold pressing, Soxhlet, and supercritical fluid extraction) in isolating oil from cherry seeds was compared. The extraction yield, fatty acids profile, tocopherols yield, and antioxidant activity of the oils were examined. Supercritical fluid extraction (SFE) achieved the highest yield and exhibited better or comparable antioxidant potential compared to traditional techniques. The comparison between modern and conventional extractions provides insights into their pros and cons for industrial application.