Serum B-cell activating factor and lung ultrasound B-lines in connective tissue disease related interstitial lung disease

ObjectiveTo investigate the role of serum B-cell activating factor (BAFF) and lung ultrasound (LUS) B-lines in connective tissue disease related interstitial lung disease (CTD-ILD), and their association with different ILD patterns on high resolution computed tomography (HRCT) of chest. MethodsWe measured the levels of BAFF and KL-6 by ELISA in the sera of 63 CTD-ILD patients [26 with fibrotic ILD (F-ILD), 37 with non-fibrotic ILD (NF-ILD)], 30 CTD patients without ILD, and 26 healthy controls. All patients underwent chest HRCT and LUS examination. ResultsSerum BAFF levels were significantly higher in CTD patients compared to healthy subjects (617.6 +/- 288.1 pg/ml vs. 269.0 +/- 60.4 pg/ml, p < 0.01). BAFF concentrations were significantly different between ILD group and non-ILD group (698.3 +/- 627.4 pg/ml vs. 448.3 +/- 188.6 pg/ml, p < 0.01). In patients with ILD, BAFF concentrations were significantly correlated with B-lines number (r = 0.37, 95% CI 0.13-0.56, p < 0.01), KL-6 level (r = 0.26, 95% CI 0.01-0.48, p < 0.05), and Warrick score (r = 0.33, 95% CI 0.09-0.53, p < 0.01), although all correlations were only low to moderate. B-lines number correlated with Warrick score (r = 0.65, 95% CI 0.48-0.78, p < 0.01), and KL-6 levels (r = 0.43, 95% CI 0.21-0.61, p < 0.01). Patients with F-ILD had higher serum BAFF concentrations (957.5 +/- 811.0 pg/ml vs. 516.1 +/- 357.5 pg/ml, p < 0.05), KL-6 levels (750.7 +/- 759.0 U/ml vs. 432.5 +/- 277.5 U/ml, p < 0.05), B-lines numbers (174.1 +/- 82 vs. 52.3 +/- 57.5, p < 0.01), and Warrick score (19.9 +/- 4.6 vs. 13.6 +/- 3.4, p < 0.01) vs. NF-ILD patients. The best cut-off values to separate F-ILD from NF-ILD using ROC curves were 408 pg/ml for BAFF (AUC = 0.73, p < 0.01), 367 U/ml for KL-6 (AUC = 0.72, p < 0.05), 122 for B-lines number (AUC = 0.89, p < 0.01), and 14 for Warrick score (AUC = 0.87, p < 0.01) respectively. ConclusionSerum BAFF levels and LUS B-lines number could be useful supportive biomarkers for detecting and evaluating the severity and/or subsets of CTD-ILD. If corroborated, combining imaging, serological, and sonographic biomarkers might be beneficial and comprehensive in management of CTD-ILD.

Automated summary

This study investigated the role of serum B-cell activating factor (BAFF) and lung ultrasound (LUS) B-lines in connective tissue disease related interstitial lung disease (CTD-ILD). The results showed that serum BAFF levels were correlated with B-lines number, KL-6 level, and Warrick score in ILD patients. Patients with fibrotic ILD had higher BAFF concentrations and B-lines numbers. ROC curve analysis determined the optimal cut-off values to distinguish fibrotic ILD from non-fibrotic ILD.