The Troublesome Ticks Research Protocol: Developing a Comprehensive, Multidiscipline Research Plan for Investigating Human Tick-Associated Disease in Australia

In Australia, there is a paucity of data about the extent and impact of zoonotic tick-related illnesses. Even less is understood about a multifaceted illness referred to as Debilitating Symptom Complexes Attributed to Ticks (DSCATT). Here, we describe a research plan for investigating the aetiology, pathophysiology, and clinical outcomes of human tick-associated disease in Australia. Our approach focuses on the transmission of potential pathogens and the immunological responses of the patient after a tick bite. The protocol is strengthened by prospective data collection, the recruitment of two external matched control groups, and sophisticated integrative data analysis which, collectively, will allow the robust demonstration of associations between a tick bite and the development of clinical and pathological abnormalities. Various laboratory analyses are performed including metagenomics to investigate the potential transmission of bacteria, protozoa and/or viruses during tick bite. In addition, multi-omics technology is applied to investigate links between host immune responses and potential infectious and non-infectious disease causations. Psychometric profiling is also used to investigate whether psychological attributes influence symptom development. This research will fill important knowledge gaps about tick-borne diseases. Ultimately, we hope the results will promote improved diagnostic outcomes, and inform the safe management and treatment of patients bitten by ticks in Australia.

Automated summary

There is a lack of data about tick-related illnesses in Australia, especially the Debilitating Symptom Complexes Attributed to Ticks (DSCATT). This research plan aims to investigate the causes, pathophysiology, and clinical outcomes of tick-associated disease in Australia, using prospective data collection, matched control groups, and integrative data analysis. The study includes laboratory analyses, such as metagenomics, to explore potential pathogen transmission during tick bites, as well as multi-omics technology to investigate links between immune responses and disease causations. Psychometric profiling will also be used to examine the influence of psychological attributes on symptom development. The research will contribute to a better understanding of tick-borne diseases and improve diagnostic outcomes and treatment for patients bitten by ticks in Australia.