Dietary Supplementation with Botanical Blends Modified Intestinal Microbiota and Metabolomics of Weaned Pigs Experimentally Infected with Enterotoxigenic Escherichia coli

The objective of this study was to investigate supplementation of botanical blends (BB) comprised of 0.3% capsicum oleoresin and 12% garlic oil on gut microbiota and metabolomic profiles in serum and ileal mucosa of Escherichia coli infected pigs. Sixty weaned pigs were assigned to one of five treatments: negative control (CON-), positive control (CON+), dietary supplementation of 100 ppm BB1, 50 or 100 ppm BB2. All pigs, except CON-, were orally inoculated with 10(10) CFU F18 ETEC/3-mL dose for 3 consecutive days after 7 d adaption. Feces, ileal digesta and cecal content were collected for 16S rRNA amplicon sequencing. Serum and ileal mucosa underwent primary metabolomics analysis. Supplementing 100 ppm BB1 increased (p < 0.05) relative abundances of Enterobacteriaceae and Escherichia-Shigella in ileum, and the relative abundances of Bacteroidota and Prevotellaceae in cecum than CON+ on d 5 post-inoculation (PI). Supplementing 100 ppm BB2 upregulated serum pinitol on d 4 PI and serum cholesterol and aminomalonic acids on d 21 PI, while supplementing 50 ppm BB2 reduced asparagine in ileal mucosa on d 5 PI than CON+. Supplementation with botanical blends modulated ileal and cecal microbiota and serum metabolomics profiles in weaned pigs under Escherichia coli challenge.

Automated summary

The objective of this study was to investigate the effects of supplementation of botanical blends (BB) on the gut microbiota and metabolomic profiles in pigs infected with Escherichia coli. The results showed that supplementing 100 ppm BB1 increased the relative abundances of Enterobacteriaceae and Escherichia-Shigella in the ileum, and the relative abundances of Bacteroidota and Prevotellaceae in the cecum. Supplementing 100 ppm BB2 upregulated serum pinitol on day 4 post-inoculation and serum cholesterol and aminomalonic acids on day 21 post-inoculation. Addition of 50 ppm BB2 reduced asparagine in the ileal mucosa on day 5 post-inoculation.