Gender Effects of Dioecious Plant Populus cathayana on Fungal Community and Mycorrhizal Distribution at Different Arid Zones in Qinghai, China

Dioecious plants have a wide distribution in nature and gender effect may cause significant alterations in rhizosphere fungal community and soil properties. However, little is known regarding changes in response to dioecious plants. This study aimed to investigate the effects that the dioecious plant, Populus cathayana, and regions of different arid levels have on the fungal community, mycorrhizal distribution, soil enzymatic activities, and nutrient contents. This study characterized fungal and soil factors from the rhizosphere of the dioecious plant Populus cathayana located in the semi-humid regions (Chengguan), semi-arid regions (Sining, Haiyan) and arid regions (Ulan, Chaka). Rhizosphere soil was collected from each site and gender, and the total fungal genomic DNA was extracted. DNA amplicons from fungal ITS region were generated and subjected to Illumina Miseq sequencing. A total of 5 phyla, 28 classes, 92 orders, 170 families, and 380 genuses were observed. AMF distribution peaked at Chaka, which did not conform to the trend. Gender had significant effects on fungal communities: there were obvious differences in fungal OTUs between genders. Alpha diversity raised at first and then decreased. RDA results showed available P, available K, pH, ALP activity, ammonium N, EC, water content and catalase activity were the key contributors in sample areas. Our results suggested potential interaction effects between plant gender and fungal community.

Automated summary

This study aimed to investigate the effects of dioecious plants and regions of different arid levels on fungal communities, mycorrhizal distribution, soil enzymatic activities, and nutrient contents. The study found that mycorrhizal distribution peaked at Chaka, which was contrary to the expected results. Gender had significant effects on fungal communities, with clear differences between genders. The key contributors in sample areas were found to be available P, available K, pH, ALP activity, ammonium N, EC, water content, and catalase activity.