4.7 Article

Utilizing Proteomic Approach to Analyze Potential Antioxidant Proteins in Plant against Irradiation

Journal

ANTIOXIDANTS
Volume 11, Issue 12, Pages -

Publisher

MDPI
DOI: 10.3390/antiox11122498

Keywords

plants against stress; anti-oxidant; gamma-ray irradiation; flavonoid; reactive oxygen species; plant defense response

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Gamma-ray irradiation on mung beans resulted in decreased malondiadehyde concentration, increased flavonoids and DPPH radical scavenging activity levels, and the identification of antioxidant proteins.
Gamma-ray irradiation is an effective and clean method of sterilization by inactivating microorganisms. It can also be applied to induce anti-oxidants for future application. In this study, the mung bean (Vigna radiata) was exposed to gamma-ray irradiation under the dose of 0, 5 or 10 kGy. With increasing irradiation doses, the concentrations of malondiadehyde decreased while the levels of total flavonoids and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity increased. It has been shown that consuming flavonoids can provide protective effects. In addition, proteomic analysis identified several proteins having anti-oxidant activities in the 5 kGy irradiated group. These proteins are Apocytochrome f, Systemin receptor SR 160, DELLA protein DWARF8, DEAD-box ATP-dependent RNA helicase 9, zeta-carotene desaturase (ZDS), and Floral homeotic protein AGAMOUS. Our findings indicate that plants contain a variety of phytochemicals and antioxidant proteins which may effectively prevent oxidative stress caused by irradiated peroxidation.

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