Journal
ANTIOXIDANTS
Volume 11, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/antiox11122495
Keywords
Mexican Gordolobo; supercritical CO2 extraction; phytochemicals; antioxidants; fatty acids; biomass valorization
Funding
- European Union's Horizon 2020 research and innovation program [778168]
- Fundacao para a Ciencia e Tecnologia [UIDB/00100/2020, UIDP/00100/2020]
- FCT [LA/P/0056/2020]
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This study investigates the potential of using the biomass of Gnaphalium viscosum for extracting phytochemicals. The results demonstrate that G. viscosum has significant potential for valorization, as it contains various highly potent antioxidants.
This work investigates the prospects for exploitation of Gnaphalium viscosum (Kunth) abundant but with limited applications till present biomass. The feasibility of traditional techniques (two-phase solvent, and the benchmark Soxhlet extraction) and supercritical extraction without/with a cosolvent at T = 40-60 degrees C and p = 30-50 MPa was examined to explore the possibility of recovering phytochemicals from G. viscosum leaves, flowers and stems. The efficiency of the techniques was assessed and compared based on yield, influence of solvents used, total phenolic content and antioxidant activity of the extracts. Phenolics of different complexities were identified and quantified by applying LC (LC-MS/MS, and LC-HRAM), while the fatty acid profile was determined by GC-FID. The results of this extensive study demonstrated the huge valorization potential and prospects of G. viscosum, since highly potent antioxidants such as kaempferol, kaempferol-3-O-beta-d-glucoside (astragalin), and chlorogenic acid were ascertained in considerable amounts. Furthermore, for the first time, the presence of leontopodic acid, a greatly substituted derivative of glucaric acid, was detected in the species.
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