Selective detection of chloramphenicol in milk based on a molecularly imprinted polymer-surface-enhanced Raman spectroscopic nanosensor

In this study, a novel hybrid molecularly imprinted polymer (MIP) with a surface-enhanced Raman spectroscopy (SERS) sensor was proposed for the detection of chloramphenicol in milk. The MIP was prepared by precipitation polymerization, and the template molecule was extracted by ultrasonic wave. The polymer was placed in a sodium citrate reduction reaction to prepare the chloramphenicol-MIP-Au substrate. The optimal detection conditions were carried out in a mixed solution of methanol and water (1:9). The sensor can be used to detect chloramphenicol in milk samples with a detection limit of 0.1 mu g/ml. The chloramphenicol-MIP-Au substrate served not only as an enrichment medium but also as an enhancement substrate base for SERS detection. Experimental results showed that the MIP hybrid as a SERS platform owned potential for simplifying the detection step and highly selective detection of chloramphenicol in milk. Copyright (c) 2016 John Wiley & Sons, Ltd.