Journal
AGRONOMY-BASEL
Volume 12, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/agronomy12122922
Keywords
Glycine max; Phytophthora sojae; microRNA; RNA sequencing; resistance
Categories
Funding
- Core Technology Development for Breeding Program of Jiangsu Province [JBGS-2021-059, JBGS-2021-014]
- National Key R&D Program of China [2021YFD1201605]
- Fundamental Research Funds for the Central Universities [KYZZ2022003]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX (20) 2015]
- National Natural Science Foundation of China [32072082, 31301340]
- China Agriculture Research System of MOF and MARA
- Program for Changjiang Scholars and Innovative Research Teams in Universities [PCSIRT_17R55]
- Jiangsu Collaborative Innovation Center for Modern Crop Production
- Cyrus Tang Innovation Center for Seed Industry
Ask authors/readers for more resources
This study analyzed the expression profiles of soybean miRNAs before and after infection with Phytophthora sojae and identified miRNAs related to resistance and susceptibility. Potential target genes of these differentially expressed miRNAs were predicted. The findings contribute to understanding the disease resistance mechanism in soybean.
Phytophthora root rot, caused by Phytophthora sojae (P. sojae), is one of the most devastating diseases limiting soybean production worldwide. microRNAs (miRNAs) play major roles in regulating plant defense against pathogens. To understand the roles of soybean miRNAs during P. sojae infection, we analyzed four small RNA libraries from two soybean germplasms before and after P. sojae isolate JS08-12 infection. The cultivar Nannong 10-1 was resistant to JS08-12, whereas the 06-070583 line was susceptible to JS08-12. In total, 528 known and 555 putative novel miRNAs in soybean were identified from 97 million reads; 74 known miRNAs and 75 novel miRNAs that might be specifically related to Nannong10-1 responses to P. sojae; and 55 known and 43 novel miRNAs expressed before and after infection in the susceptible line 06-070583. qRT-PCR provided similar miRNA expression patterns to those obtained by the small-RNA sequencing of the four libraries. Then, the potential target genes of these differentially expressed miRNA were predicted, which encoded transcriptional factors, resistance proteins and transporters. Finally, we focused on the targets of the three legume-specific miRNAs (gma-miR1508, gma-miR1509, and gma-miR1510) and charted the miRNA-target interactions and networks based on the published degradome data.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available