Immunodetection of P2X2 Receptor in Enteric Nervous System Neurons of the Small Intestine of Pigs

Simple Summary The enteric nervous system (ENS) is responsible for regulating the main functions of the intestines, i.e., absorption, secretion, peristalsis and sensation. The ENS is composed of nerve cells that form clusters in the form of ganglia arranged in nerve plexuses. Individual ganglia communicate through a system of nerve fibres. Each nerve cell or nerve fibre contains biologically active substances exerting a specific effect on the ENS. One such substance is extracellular adenosine 5 '-triphosphate (ATP), which by activating purinoreceptors (including P2X2 receptors) may affect the functioning of the ENS. Previous research on the P2X2 receptor has focused mainly on laboratory animals, while little is known about this receptor in large mammals. Therefore, our research concerned the presence of the P2X2 receptor in the ENS of the small intestine of the pig and whether it coexists with the biologically active substances included in standard analyses in the ENS. The research showed that the P2X2 receptor is present in the neurons of the small intestine and coexists with some of the biologically active substances tested. These findings may suggest that the P2X2 receptor, activated by ATP, may exert an influence on the function of the small intestinal ENS of the pig. Extracellular adenosine 5 '-triphosphate (ATP) is one of the best-known and frequently studied neurotransmitters. Its broad spectrum of biological activity is conditioned by the activation of purinergic receptors, including the P2X2 receptor. The P2X2 receptor is present in the central and peripheral nervous system of many species, including laboratory animals, domestic animals, and primates. However, the distribution of the P2X2 receptor in the nervous system of the domestic pig, a species increasingly used as an experimental model, is as yet unknown. Therefore, this study aimed to determine the presence of the P2X2 receptor in the neurons of the enteric nervous system (ENS) of the pig small intestine (duodenum, jejunum, and ileum) by the immunofluorescence method. In addition, the chemical code of P2X2-immunoreactive (IR) ENS neurons of the porcine small intestine was analysed by determining the coexistence of selected neuropeptides, i.e., vasoactive intestinal polypeptide (VIP), substance P (sP), and galanin. P2X2-IR neurons were present in the myenteric plexus (MP), outer submucosal plexus (OSP), and inner submucosal plexus (ISP) of all sections of the small intestine (duodenum, jejunum, and ileum). From 44.78 +/- 2.24% (duodenum) to 63.74 +/- 2.67% (ileum) of MP neurons were P2X2-IR. The corresponding ranges in the OSP ranged from 44.84 +/- 1.43% (in the duodenum) to 53.53 +/- 1.21% (in the jejunum), and in the ISP, from 53.10 +/- 0.97% (duodenum) to 60.57 +/- 2.24% (ileum). Immunofluorescence staining revealed the presence of P2X2-IR/galanin-IR and P2X2-IR/VIP-IR neurons in the MP, OSP, and ISP of the sections of the small intestine. The presence of sP was not detected in the P2X2-IR neurons of any ganglia tested in the ENS. Our results indicate for the first time that the P2X2 receptor is present in the MP, ISP, and OSP neurons of all small intestinal segments in pigs, which may suggest that its activation influences the action of the small intestine. Moreover, there is a likely functional interaction between P2X2 receptors and galanin or VIP, but not sP, in the ENS of the porcine small intestine.

Automated summary

The study showed the presence of the P2X2 receptor in the neurons of the small intestine of pigs, suggesting its potential influence on the function of the small intestinal ENS. P2X2 receptor presence was observed in the myenteric plexus, outer submucosal plexus, and inner submucosal plexus neurons of all small intestinal segments in pigs, along with coexistence with certain biologically active substances.