Flubendiamide Resistance and Its Mode of Inheritance in Tomato Pinworm Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is the major pest of tomato crops in Pakistan. Insecticides are commonly used for the management of this insect-pest. To develop a better insecticide resistance management strategy and evaluate the risk of resistance evolution, a field collected population of the tomato pinworm was selected with flubendiamide in the laboratory. We investigated the genetics of flubendiamide resistance and concentration-mortality response to other insecticides by selecting a field strain of tomato pinworm with commercial flubendiamide formulation. Tuta absoluta was reciprocally crossed with resistant strain (Fluben-R) and was selected up to 13 generations, while F-1 progeny was back-crossed with resistant parent (Fluben-R). The results of LC50 and Resistance Ratio (RR) demonstrated a higher resistance developed in field and laboratory-selected strains (G(2) and G(13), respectively). Field-collected and laboratory-selected (Fluben-R) strains demonstrated higher intensity of concentration-mortality response against chlorantraniliprole, thiamethoxam, permethrin, abamectin and tebufenozide compared to susceptible ones. Based on the overlapping of 95% FL, it demonstrated significant differences, revealing that it was not sex linked (autosomal) with no maternal effects. The backcross analysis of the F-1 x resistant parent resulting in significant differences at all concentrations suggests that resistance is controlled by more than one factor; the null hypothesis was rejected and inheritance was under polygenic control. Resistance progression from 38 to 550 folds demonstrated that T. absoluta can develop a higher level of resistance to flubendiamide. Concentration-mortality response experiments demonstrated that the LC50 of some tested insecticides was higher for field-collected and laboratory-selected strains, suggesting that resistance mechanisms should be studied at a molecular level for better understanding. These results could be helpful to design resistance management strategies against the tomato pinworm.

Automated summary

The tomato pinworm, a major pest in tomato crops in Pakistan, was studied for its resistance to insecticides. The researchers found that a field collected population of the pinworm showed higher resistance levels after being selected with a specific insecticide. They also discovered that the resistance was controlled by multiple factors and that the resistance level can increase significantly over time. These findings provide valuable insights for developing resistance management strategies.