Journal
CELLS
Volume 11, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/cells11233796
Keywords
hiPSC; motor neurons; small molecules; transcription factors; movement disorders
Categories
Ask authors/readers for more resources
This article discusses the generation of motor neurons (MNs) from hiPSCs and focuses on two approaches: induction by small molecules and induction by lentiviral delivery of transcription factors. The challenges in modeling neurological diseases using iPSC-derived neurons are identified, including obtaining high purity and yield of neurons, achieving full maturation in long-term culture, and culturing neurons more physiologically to maximize relevance to in vivo conditions.
Generation of motor neurons (MNs) from human-induced pluripotent stem cells (hiPSCs) overcomes the limited access to human brain tissues and provides an unprecedent approach for modeling MN-related diseases. In this review, we discuss the recent progression in understanding the regulatory mechanisms of MN differentiation and their applications in the generation of MNs from hiPSCs, with a particular focus on two approaches: induction by small molecules and induction by lentiviral delivery of transcription factors. At each induction stage, different culture media and supplements, typical growth conditions and cellular morphology, and specific markers for validation of cell identity and quality control are specifically discussed. Both approaches can generate functional MNs. Currently, the major challenges in modeling neurological diseases using iPSC-derived neurons are: obtaining neurons with high purity and yield; long-term neuron culture to reach full maturation; and how to culture neurons more physiologically to maximize relevance to in vivo conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available