Journal
CELLS
Volume 11, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/cells11243955
Keywords
polylaminin; laminin 521; iPSC; cell expansion; pluripotency
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Laminins (LNs) are important in cell-ECM interactions. PolyLN521, a self-polymerizing coating, can promote large-scale expansion of human iPSCs without affecting cell stability or pluripotency.
Laminins (LNs) play a central role in the self-assembly and maintenance of basement membranes and are involved in critical interactions between cells and other extracellular matrix (ECM) proteins. Among the defined, xeno-free ECM culture matrices, LNs-namely LN521-have emerged as promising coating systems for the large-scale expansion of induced pluripotent stem cells (iPSCs). The biologic activity of LNs is enhanced by their acidification-induced self-polymerization into a cell-associated network called polylaminin (polyLN), which can recapitulate the native-like polymeric array in a cell-free system. Here, we show for the first time to our knowledge that polyLN521 displays a native-like hexagonal-like structure and that, at basal and low concentrations, it permits the large-scale expansion of human iPSCs. Human iPSCs expanded with polyLN521 maintained the pluripotent state and showed no impairment of karyotype stability or telomere length. These results suggest that low-concentration polyLN521 is a stable and cost-effective coating for large-scale iPSC expansion.
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