4.8 Article

Crowder-directed interactions and conformational dynamics in multistimuli-responsive intrinsically disordered protein

Journal

SCIENCE ADVANCES
Volume 8, Issue 51, Pages -

Publisher

AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abq2202

Keywords

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Funding

  1. Australian Research Council?s Discovery Projects funding scheme [DP160101267]
  2. ANSTO beamtime awards [NDF6169, P6170]
  3. Australian Institute of Nuclear Science and Engineering Postgraduate Research award
  4. National Collaborative Research Infrastructure Strategy, an initiative of the Australian Government

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This study reveals the impact of crowding on the dynamic conformation of intrinsically disordered proteins (IDPs). Through experimental methods and model construction, researchers found that crowding can induce specific conformational changes in IDPs. This study is of great importance for a better understanding of the interactions and structural dynamics of IDPs in crowded environments.
The consequences of crowding on the dynamic conformational ensembles of intrinsically disordered proteins (IDPs) remain unresolved because of their ultrafast motion. Here, we report crowder-induced interactions and conformational dynamics of a prototypical multistimuli-responsive IDP, Rec1-resilin. The effects of a range of crowders of varying sizes, forms, topologies, and concentrations were examined using spectroscopic, spectro-fluorimetric, and contrast-matching small-and ultrasmall-angle neutron scattering investigation. To achieve sufficient neutron contrast against the crowders, deuterium-labeled Rec1-resilin was biosynthesized successfully. Moreover, the ab initio shape reconstruction approach was used to obtain three-dimensional models of the conformational assemblies. The IDP revealed crowder-specific systematic extension and compaction with the level of macromolecular crowding. Last, a robust extension-contraction model has been postulated to capture the fundamental phenomena governing the observed behavior of IDPs. The study provides insights and fresh perspectives for understanding the interactions and structural dynamics of IDPs in crowded states.

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