Journal
SCIENCE ADVANCES
Volume 8, Issue 45, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.add3221
Keywords
-
Categories
Funding
- NIH [R01NS116716, R01AI153332, R01AI153185, NIHT32DK120521]
- Department of Veteran Affairs Award [BX002711]
Ask authors/readers for more resources
In this study, a murine model of Group B Streptococcus (GBS) diabetic wound infection was developed and it was demonstrated through dual RNA sequencing that GBS infection triggers an inflammatory response. GBS adapts to this hyperinflammatory environment by up-regulating virulence factors and mutations in the covR gene were found to affect GBS hemolysin/pigmentation production. This foundational study provides insight into the pathogenesis of GBS diabetic wound infections.
Diabetic wounds have poor healing outcomes due to the presence of numerous pathogens and a dysregulated immune response. Group B Streptococcus (GBS) is commonly isolated from diabetic wound infections, but the mechanisms of GBS virulence during these infections have not been investigated. Here, we develop a murine model of GBS diabetic wound infection and, using dual RNA sequencing, demonstrate that GBS infection triggers an inflammatory response. GBS adapts to this hyperinflammatory environment by up-regulating virulence factors including those known to be regulated by the two-component system covRS, such as the surface protein pbsP, and the cyl operon, which is responsible for hemolysin/pigmentation production. We recover hyperpigmented/hemolytic GBS colonies from the murine diabetic wound, which we determined encode mutations in covR. We further demonstrate that GBS mutants in cylE and pbsP are attenuated in the diabetic wound. This foundational study provides insight into the pathogenesis of GBS diabetic wound infections.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available