Functionalizing DNA origami to investigate and interact with biological systems

DNA origami has emerged as a powerful method to generate DNA nanostructures with dynamic properties and nanoscale control. These nanostructures enable complex biophysical studies and the fabrication of next-generation therapeutic devices. For these applications, DNA origami typically needs to be functionalized with bioactive ligands and biomacromolecular cargos. Here, we review methods developed to functionalize, purify and characterize DNA origami nanostructures. We identify remaining challenges such as limitations in functionalization efficiency and characterization. We then discuss where researchers can contribute to further advance the fabrication of functionalized DNA origami.

Automated summary

DNA origami is a powerful method for generating DNA nanostructures with dynamic properties and nanoscale control. This review focuses on the methods developed for functionalizing, purifying, and characterizing these nanostructures. The remaining challenges and potential areas of advancement in the fabrication of functionalized DNA origami are also discussed.