Exploring the In-Vitro Antibacterial Activity and Protein (Human Serum Albumin, Human Hemoglobin and Lysozyme) Interaction of Hexagonal Silver Nanoparticle Obtained from Wood Extract of Wild Cherry Shrub

Here, the silver nanoparticles were synthesized using aqueous wood extract of cerasus macrocarpa (wild Cherry) shrub. This green synthesis method was both eco-friendly and straightforward. The nanoparticles were characterized using FT-IR, zeta potential, ultraviolet-visible, TEM, and SEM-EDX analysis. Green synthesized AgNPs demonstrated effective antibacterial activity against gram-positive and gram-negative bacteria (S. aureus and E. coli). Furthermore, fluorometric, circular dichroism and UV-vis were used to study the interaction of green synthesized AgNPs with Human serum albumin (HSA (, Human hemoglobin (HHb), and Lysozyme (Lys) in simulated physiological conditions. The studies of interaction with HSA, HHb and Lys show good binding affinity for green synthesized silver nanoparticles; the binding affinity was found in the order Lys>HSA>HHb at 298.15 K. The results show that the native conformation of HSA, HHb, and Lys was preserved at the secondary structure level, which is crucial in providing insights into the side effects of newly synthesized drugs on their carriers. The fluorescence data represented that green synthesized AgNPs quench the intrinsic fluorescence of proteins through a static quenching procedure.

Automated summary

The silver nanoparticles were synthesized using a green method with aqueous wood extract of cerasus macrocarpa shrub. These nanoparticles exhibited strong antibacterial activity against both gram-positive and gram-negative bacteria. The interaction between the green synthesized AgNPs and proteins in simulated physiological conditions was studied, revealing good binding affinity and the preservation of protein conformation.