Multifunctional Nanoparticle Platform for Surface Accumulative Nucleic Acid Amplification and Rapid Electrochemical Detection: An Application to Pathogenic Coronavirus

Article Biophysics

SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Yixuan Wu et al.

Summary: The AuNDS-based SERS-PCR detection method can shorten the diagnosis time by reducing the number of cycles needed, compared to traditional RT-PCR, with lower target DNA concentration requirements.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Multidisciplinary Sciences

Surface-ligand-induced crystallographic disorder-order transition in oriented attachment for the tuneable assembly of mesocrystals

Bum Chul Park et al.

Summary: This study investigates the control of crystallographic alignment in nanomaterials through the influence of surface ligands on magnetite mesocrystals. The authors found that different surface ligands can lead to different alignment variations, which are guided by surface energy reduction and surface deformation. The alignment-dependent magnetic interactions between building blocks also affect the overall magnetic properties of mesocrystals and their chains.

NATURE COMMUNICATIONS (2022)

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Review Biochemical Research Methods

Recent Developments in Innovative Magnetic Nanoparticles-Based Immunoassays: From Improvement of Conventional Immunoassays to Diagnosis of COVID-19

Yeonjeong Ha et al.

Summary: During the ongoing COVID-19 pandemic, there is an urgent need for point-of-care (POC) detection methods with high sensitivity and rapid detection time. Magnetic nanoparticles (MNPs) are attractive materials that can enhance the sensitivity and reduce the detection time of conventional immunoassays. This review discusses recent advances in different types of MNPs-based immunoassays and their advantages and strategies. Furthermore, the use of MNPs for COVID-19 diagnosis through color development is also introduced, demonstrating their potential as common tools for on-site diagnosis.

BIOCHIP JOURNAL (2022)

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Article Nanoscience & Nanotechnology

CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

Chenshuo Wu et al.

Summary: This study proposes a novel electrochemical CRISPR sensing method for the detection of SARS-CoV-2 variants. The method offers rapid, highly sensitive, and specific detection without the need for nucleic acid amplification assays.

NANO-MICRO LETTERS (2022)

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Review Chemistry, Multidisciplinary

Advanced Nanomaterials for Preparedness Against (Re-)Emerging Viral Diseases

Eunjung Kim et al.

Summary: While COVID-19 is currently causing a global pandemic, the emergence of unknown pathogens such as Disease X is a serious concern for the future. Utilizing advanced nanotechnology to address the challenges posed by emerging pathogens is crucial for global public health preparedness.

ADVANCED MATERIALS (2021)

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Review Chemistry, Physical

Diagnostics for SARS-CoV-2 infections

Bhavesh D. Kevadiya et al.

Summary: The outbreak of SARS-CoV-2 causing COVID-19 has led to diverse and severe symptoms, making accurate diagnosis a key challenge. Rapid diagnosis and treatment measures are crucial in controlling the spread of the virus. The development of diagnostic tools, including nanomaterial-based ones, is essential in the fight against the pandemic.

NATURE MATERIALS (2021)

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Article Chemistry, Analytical

Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP

Yaqin Zhang et al.

Summary: The development of a gold nanoparticle-based visual assay combined with CRISPR/Cas12a-assisted RT-LAMP for rapid and sensitive detection of SARS-CoV-2 shows promise in improving COVID-19 screening efforts. This method is easy to operate and has high-throughput testing capability, with potential applications in screening suspected individuals in various settings.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Chemistry, Analytical

A one-step, one-pot CRISPR nucleic acid detection platform (CRISPR-top): Application for the diagnosis of COVID-19

Shijun Li et al.

Summary: CRISPR-top is a novel diagnostic method that integrates target pre-amplification and CRISPR-mediated detection in one reaction mixture for SARS-CoV-2 detection. This simple, rapid, accurate, and highly sensitive method can return results within 60 minutes, making it suitable for clinical and resource-challenged settings.

TALANTA (2021)

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Article Multidisciplinary Sciences

Rapid electrochemical detection of coronavirus SARS-CoV-2

Thanyarat Chaibun et al.

Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.

NATURE COMMUNICATIONS (2021)

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Article Chemistry, Analytical

Rapid and sensitive triple-mode detection of causative SARS-CoV-2 virus speci fi c genes through interaction between genes and nanoparticles

Yakun Gao et al.

Summary: This article introduces a triple-mode biosensor based on AuNPs, which can rapidly and selectively detect viral RNA, achieving femtomole level detection limits in absorbance, fluorescence, and SERS modes. The sensor effectively minimizes false negative/positive readings of SARS-CoV-2.

ANALYTICA CHIMICA ACTA (2021)

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Review Chemistry, Medicinal

A Closer Look into FDA-EUA Approved Diagnostic Techniques of Covid-19

Hyunju Oh et al.

Summary: The review highlights the global pandemic of the 2019 coronavirus disease (COVID-19) and the approval status of diagnostic kits, emphasizing the importance of selecting appropriate products to maximize testing efficiency. Understanding differences and developing new diagnostic techniques to optimize current testing options are essential.

ACS INFECTIOUS DISEASES (2021)

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Article Biochemical Research Methods

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

Maha Alafeef et al.

Summary: This protocol presents a novel approach for rapid detection of COVID-19 without the need for RNA extraction, using nucleic acid amplification and plasmonic sensing technology. The test demonstrates high accuracy, sensitivity (>98.4%), specificity (>96.6%) and a detection limit of 10 copies/mu L.

NATURE PROTOCOLS (2021)

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Article Chemistry, Multidisciplinary

CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

Yiren Cao et al.

Summary: The trans-cleavage activity of target-activated CRISPR/Cas12a released an RNA crosslinker from a molecular transducer, aiding in the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a allowed for visual detection of the N gene and E gene of SARS-CoV-2 in 45 minutes.

CHEMICAL COMMUNICATIONS (2021)

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Article Chemistry, Multidisciplinary

Rapid and ultrasensitive electrochemical detection of circulating tumor DNA by hybridization on the network of gold-coated magnetic nanoparticles

Dongfei Chen et al.

Summary: An ultrasensitive electrochemical biosensor based on DNA-Au@MNPs has been developed for rapid detection of ctDNA in whole blood, with an ultralow detection limit and broad dynamic range. This sensor provides a promising method for achieving a 20-minute response time and minimally invasive early cancer diagnosis.

CHEMICAL SCIENCE (2021)

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Article Infectious Diseases