Journal
ACS SENSORS
Volume 7, Issue 11, Pages 3470-3480Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.2c01808
Keywords
surface-enhanced Raman scattering; SERS-LFA; SARS-CoV-2; portable Raman reader; immunoassay
Funding
- National Research Foundation of Korea [2019R1A2C3004375, 2020R1A5A1018052, 2021M3E5E3080379]
- government-wide R&D fund for the research of infectious diseases in Korea [HG18C0062]
- KRIBB Research Initiative Program [1711134081]
- National Nature Science Foundation of China [21976209]
- National Research Foundation of Korea [2021M3E5E3080379] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to improve the detection sensitivity of commercial LFA strips. Testing 54 clinical samples, the SERS-LFA reader demonstrated a significantly reduced false-negative rate compared to commercial LFA strips.
In early 2022, the number of people infected with the highly contagious mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), called Omicron, was increasing worldwide. Therefore, several countries approved the lateral flow assay (LFA) strip as a diagnostic method for confirming SARS-CoV-2 instead of reverse transcription-polymerase chain reaction (RT-PCR), which takes a long time to generate the results. However, owing to the limitation of detection sensitivity, commercial LFA strips have high false-negative diagnosis rates for patients with low virus concentrations. Therefore, in this study, we developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to solve the sensitivity problem of the commercial LFA strip. We tested 54 clinical samples using this portable SERS-LFA reader, which generated 49 positive and 5 negative results. Out of the 49 positive results, SERS-LFA classified only 2 as false negative, while the commercial LFA classified 21 as false negative. This confirmed that the false-negative rate had significantly improved compared to that of commercial LFA strips. We believe that the proposed SERS-LFA system can be utilized as a point-of-care diagnostic system to quickly and accurately determine a virus infection that could spread significantly within a short period.
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