Journal
JOURNAL OF PROTEOMICS
Volume 143, Issue -, Pages 254-264Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2016.04.016
Keywords
Tomato; Fruit; Carbon metabolism; 14-3-3 protein; Ubiquitin ligase; Protein interactome
Categories
Funding
- Japan Society for the Promotion of Science (JSPS) [15K18819, 15H0116705, 2629218885, 2666004604]
- Cooperative Research Grant of the Plant Trans genic Design Initiative, Gene Research Center, the University of Tsukuba
- RIKEN Plant Transformation Network
- Center for Food and Medical Innovation
- Special Grant Program for Young Foreign Scientists in Basic Science (Hokkaido University Faculty of Science)
- MEXT Honors Scholarship for Privately Financed International Students
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [16J03184, 15H01167, 26292188, 26660046, 15K18819] Funding Source: KAKEN
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The 14-3-3 proteins participate in many aspects of plant physiology by interacting with phosphorylated proteins and thereby regulating target protein functions. In Arabidopsis plant, the ubiquitin ligase ATL31 controls 14-3-3 stability via both direct interaction and ubiquitination, and this consequently regulates post-germinative growth in response to carbon and nitrogen nutrient availability. Since 14-3-3 proteins regulate the activities of many key enzymes related to nutrient metabolism, one would anticipate that they should play an essential role not only in vegetative but also in reproductive tissue. Because fruit yield largely depends on carbon and nitrogen availability and their utilization, the function of 14-3-3 proteins was analyzed in tomato fruit tissue. Here, we isolated and characterized an ubiquitin ligase SIATL31 (Solyc03g112340) from tomato and demonstrated that SIATL31 has ubiquitin ligase activity as well as interaction with tomato 14-3-3 proteins, suggesting the possibility that the SIATL31 functions as an ubiquitin ligase for 14-3-3 similarly to its Arabidopsis ortholog. Furthermore, we performed proteomic analysis of 14-3-3 interacting proteins and identified 106 proteins as putative 14-3-3 targets including key enzymes for carbon metabolism and photosynthesis. This 14-3-3 interactome result and available transcriptome profile suggest a considerable yet complex role of 14-3-3 proteins in tomato fruit tissue. Biological significance: Considerable cumulative evidence exists which implies that 14-3-3 proteins are involved in the regulation of plant primary metabolism. Here we provide the first report of 14-3-3 interactome analysis and identify putative 14-3-3 targets in tomato fruit tissue, which may be highly important given the documented metabolic shifts, which occur during fruit development and ripening. These data open future research avenues by which to understand the regulation of the role of post-translational regulation in tomato fruit development. (C) 2016 Elsevier B.V. All rights reserved.
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