H2S-based fluorescent imaging for pathophysiological processes

Hydrogen sulfide (H2S), as an important endogenous signaling molecule, plays a vital role in many physiological processes. The abnormal behaviors of hydrogen sulfide in organisms may lead to various pathophysiological processes. Monitoring the changes in hydrogen sulfide is helpful for pre-warning and treating these pathophysiological processes. Fluorescence imaging techniques can be used to observe changes in the concentration of analytes in organisms in real-time. Therefore, employing fluorescent probes imaging to investigate the behaviors of hydrogen sulfide in pathophysiological processes is vital. This paper reviews the design strategy and sensing mechanisms of hydrogen sulfide-based fluorescent probes, focusing on imaging applications in various pathophysiological processes, including neurodegenerative diseases, inflammation, apoptosis, oxidative stress, organ injury, and diabetes. This review not only demonstrates the specific value of hydrogen sulfide fluorescent probes in preclinical studies but also illuminates the potential application in clinical diagnostics.

Automated summary

Hydrogen sulfide (H2S) as an important endogenous signaling molecule, has a vital role in physiological processes. Monitoring changes in hydrogen sulfide can help pre-warning and treating pathophysiological processes. Fluorescence imaging techniques can be used to observe real-time changes in analyte concentration in organisms. This review focuses on the design and sensing mechanisms of hydrogen sulfide-based fluorescent probes and their imaging applications in various pathophysiological processes. It demonstrates the value of these probes in preclinical studies and their potential application in clinical diagnostics.