Journal
JOURNAL OF PROTEOME RESEARCH
Volume 16, Issue 1, Pages 264-273Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00584
Keywords
secretome; Helicobacter pylori (H. pylori); THP-1; elongation factor Tu (EF-Tu); adherence; virulence
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Funding
- National Science Council [MOST100-2113-M-006-002-MY3, MOST102-2313-B-022-002]
- Ministry of Education, Taiwan, R.O.C. under the NCKU Project of Promoting Academic Excellence & Developing World Class Research Centers
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The secreted proteins of bacteria are usually accompanied by virulence factors, which can cause inflammation and damage host cells. Identifying the secretomes arising from the interactions of bacteria and host cells could therefore increase understanding of the mechanisms during initial pathogenesis. The present study used a host-pathogen coculture system of Helicobacter pylori and monocytes (THP-1 cells) to investigate the secreted proteins associated with initial H. pylori pathogenesis. The secreted proteins from the conditioned media from H. pylori, THP-1 cells, and the coculture were collected and analyzed using SDS-PAGE and LC-MS/MS. Results indicated the presence of 15 overexpressed bands in the coculture. Thirty-one proteins were identified-11 were derived from THP-1 cells and 20 were derived from H. pylori. A potential adherence factor from H. pylori, elongation factor-Tu (EF-Tu), was selected for investigation of its biological function. Results from confocal microscopic and flow cytometric analyses indicated the contribution of EF-Tu to the binding ability of H. pylori in THP-1. The data demonstrated that fluorescence of EF-Tu on THP-1 cells increased after the addition of the H. pylori-conditioned medium. This study reports a novel secretory adherence factor in H. pylori, EF-Tu, and further elucidates mechanisms of H. pylori adaptation for host-pathogen interaction during pathogenesis.
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