Journal
JOURNAL OF PROTEOME RESEARCH
Volume 15, Issue 8, Pages 2643-2657Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00206
Keywords
proteomics; metabolomics; Clematis terniflora DC.; leaf; ultraviolet-B irradiation; dark treatment
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Funding
- National Science Foundation of China [81274033]
- Zhejiang Provincial Natural Science Foundation of China [LZ14H280001]
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Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B irradiation with dark treatment. Metabolites related to carbohydrates, fatty acids, and amino acids and/or proteins related to stress, cell wall, and amino acid metabolism were gradually increased in response to high-level UV-B irradiation with dark treatment. On the basis of cluster analysis and mapping of proteins related to amino acid metabolism, the abundances of S-adenosylmethionine synthetase and cysteine synthase as well as 1,1-diphenyl-2-picrylhydrazyl scavenging activity were gradually increased in response to high-level UV-B irradiation with dark treatment. Furthermore, the abundance of dihydrolipoyl dehydrogenase/glutamate dehydrogenase and the content of gamma-aminobutyric acid were also increased following high-level UV -B irradiation with dark treatment. Taken together, these results suggest that high-level UV -B irradiation with dark treatment induces the activation of reactive oxygen species scavenging system and gamma-aminobutyric acid shunt pathway in leaf of C. terniflora.
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