X-ray-Induced Heating in the Vicinity of the X-ray Interaction Point

Article Multidisciplinary Sciences

Beef tallow injection matrix for serial crystallography

Ki Hyun Nam

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SCIENTIFIC REPORTS (2022)

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Article Crystallography

Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

Yongsam Kim et al.

Summary: In this study, a pink-beam serial synchrotron crystallographic experiment was conducted at the 1C beamline at the Pohang Light Source II, successfully determining lysozyme crystal structures at a high resolution of 1.7 angstrom. Different X-ray exposure positions and times were compared to analyze their impact on data collection and structure determination.

CRYSTALS (2022)

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Combination of an inject-and-transfer system for serial femtosecond crystallography

Keondo Lee et al.

Summary: Serial femtosecond crystallography (SFX) is a method that enables the determination of macromolecular crystal structures at room temperature with minimal radiation damage and provides time-resolved molecular dynamics. A combined inject-and-transfer system (BITS) method for sample delivery in SFX experiments has been introduced, allowing reliable deposition of solution samples on UV ozone-treated polyimide films. This method has been successfully applied to determine the room-temperature structure of lysozyme.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2022)

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Article Chemistry, Multidisciplinary

Radiation Damage of Polydimethylsiloxane and Polyimide by X-ray Free-Electron Laser

Keondo Lee et al.

Summary: This study investigated the radiation damage caused by an XFEL to PDMS and PI materials, commonly used in sample delivery devices. The results showed different structural damages in PDMS and PI films, providing insights for developing applications of these materials in XFEL studies.

APPLIED SCIENCES-BASEL (2022)

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Article Biochemistry & Molecular Biology

Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes

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CURRENT OPINION IN STRUCTURAL BIOLOGY (2022)

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CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

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Article Biochemistry & Molecular Biology

Room-Temperature Structure of Xylitol-Bound Glucose Isomerase by Serial Crystallography: Xylitol Binding in the M1 Site Induces Release of Metal Bound in the M2 Site

Ki Hyun Nam

Summary: The study reveals that xylitol binding to GI causes a change in the geometric coordination of metal binding sites, leading to the rearrangement of metal-binding residues at the M1 and M2 sites. This sheds light on the reason for the decreased metal-binding affinity and enhances understanding of the xylitol-bound state of SruGI for potential industrial applications.

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Article Multidisciplinary Sciences

Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison

P. Mehrabi et al.

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Article Multidisciplinary Sciences

Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography

Ki Hyun Nam et al.

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Viscous-medium-based crystal support in a sample holder for fixed-target serial femtosecond crystallography

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X-ray-Induced Heating in the Vicinity of the X-ray Interaction Point

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