Phytochemicals Identification Using GC-MS in Four Extracts of Fruit Peels and Enactment of Extracts Against Pseudomonas Aeruginosa MZ269380

Organic wastes constitute a major share of Municipal Solid Wastes. The most beneficial approach to handling such wastes is to recover the bioactive constituents by biovalorization, making full use of them in the food, pharmaceutical as well as cosmetics industry. In this paper, fruit residual waste is collected from the waste of a residential and fruit-juice centre in Purba Medinipur district of West Bengal (India). Physico-chemical analysis of the powder generated from these wastes indicates a heterogeneous nature of powder particles having a diameter ranging from 10 to 55 mu m. The moisture content of the powder was nearly 4%. Important minerals like C, K, Ca, N, O, Na, Mg, Zn, Si, P and K were detected in the powder. Four different extracts were prepared from the powder using solvents: ethanol, methanol, petroleum ether and butanol. Biochemical analysis of each extract showed high antioxidant properties. Each extraction was profiled using Gas Chromatography-Mass Spectroscopy analysis to screen the presence of functional bioactive molecules. A total of 33 molecules were identified, among which notable were N-Hexadecanoic acid; Vitamine-E; Squalene; Ergosta-7; Oleic acid; Hexadecane; 1,16-Dichloro etc. Methanol, Ethanol, Petroleum ether and Butanol extracts showed cytotoxic potential against Pseudomonas aeruginosa MZ269380 having MIC (mg/Ml) values of 19.5, 12.5, 24.5 and 8 respectively. Biocompatibility assay of each extract showed no significant damage to goat's erythrocytes at MIC concentration. Biovalorisation of fruit peels produces useful phytochemicals that can be used in the biomedical, food processing and cosmetics industries.

Automated summary

In this study, fruit peels waste was collected from a residential and fruit-juice center in Purba Medinipur district of West Bengal, India. The powder generated from these wastes was analyzed, and it was found to have heterogeneous particles with a diameter ranging from 10 to 55 μm and a moisture content of approximately 4%. Various minerals and 33 bioactive molecules were detected in the powder. Different extracts were prepared from the powder using solvents and showed high antioxidant properties. Methanol, ethanol, petroleum ether, and butanol extracts exhibited cytotoxic potential against Pseudomonas aeruginosa MZ269380, and all extracts were biocompatible with goat's erythrocytes at MIC concentration. The biovalorization of fruit peels can produce useful phytochemicals for biomedical, food processing, and cosmetics industries.