Application of the Biolog EcoPlateTM technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica

Little is known about metabolic capabilities of microbial assemblages in continental Antarctic to utilize diverse carbon sources within moss profiles consisting of live, senescent, and dead moss tissues in different stages of decomposition. In the present study, microbial metabolic diversity was determined using Biolog EcoPlateTM for samples of Bryum pseudotriquetrum/B. archangelicum complex collected at five sites located in coastal outcrops of the Lutzow-Holm Bay area (Queen Maud Land), East continental Antarctica, and effects of chemical, biological, and physical properties of the moss tissues were explored. The average well-color development, the number of substrates utilized, Simpson's diversity index and equitability of the microbial substrate utilization, and the utilization of carbohydrates, amino acids/amines, carboxylic acids, and polymers varied significantly among the five sites and among four layers of moss profiles. Principal component analysis showed that the utilization of L-asparagine, L-arginine, D-galacturonic acid, Tween 40, Tween 80, and D-mannitol mainly contributed to the variance in the microbial metabolic diversity between sites and moss profiles. The linear mixed models indicated that the relative content of recalcitrant organic components, the number of fungal species, and the water content of moss tissues affected the diversity of microbial utilization of carbon sources.

Automated summary

Little is known about the metabolic capabilities of microbial assemblages in continental Antarctic moss. In this study, microbial metabolism was analyzed using Biolog EcoPlateTM, revealing significant variations in the utilization of different carbon sources. The chemical, biological, and physical properties of the moss tissues were found to play a role in the microbial utilization of carbon sources.