4.6 Article

DNA barcoding unveils a high diversity of caddisflies (Trichoptera) in the Mount Halimun Salak National Park (West Java; Indonesia)

Journal

PEERJ
Volume 10, Issue -, Pages -

Publisher

PEERJ INC
DOI: 10.7717/peerj.14182

Keywords

Mitochondrial COI; Larval-adult association; Species boundary; Species delimitation; Freshwater; Automatic barcode gap discovery; Bayesian poisson tree processes; Assemble species by automatic partitioning; Generalized mixed yule coalescent

Funding

  1. German Federal Ministry of Education and Research (BMBF
  2. INDOBIOSYS MfN Berlin)
  3. Indonesian DIPA [16GW0111K]
  4. BMBF (INDOBIOSYS ZSM Munich) [3400.003.050.I, 079.01.2.017148 2016, 3400.010.005.061B, 16GW0112]
  5. Open Access Fund of the Leibniz Association [079.01.2.017148 2015]

Ask authors/readers for more resources

This study assessed the Trichoptera diversity in and around Mount Halimun Salak National Park in West Java, Indonesia using molecular and morphological approaches. The results revealed a high diversity of Trichoptera in the area and demonstrated the potential of Trichoptera as bioindicators for water quality assessment. The study also highlighted the importance of improving DNA barcode reference libraries for Trichoptera in the Oriental region.
Background: Trichoptera are one of the most diverse groups of freshwater insects worldwide and one of the main bioindicators for freshwater quality. However, in many areas, caddisflies remain understudied due to lack of taxonomic expertise. Meanwhile, globally increasing anthropogenic stress on freshwater streams also threatens Trichoptera diversity.Methods: To assess the Trichoptera diversity of the area within and around the Mount Halimun Salak National Park (MHSNP or Taman Nasional Gunung Halimun Salak) in West Java (Indonesia), we conducted a molecular-morphological study on Trichoptera diversity using larvae from a benthic survey and adults from hand-netting. In addition to morphological identification, we applied four different molecular taxon delimitation approaches (Generalized Mixed Yule Coalescent, Bayesian Poisson Tree Processes, Automatic Barcode Gap Discovery and Assemble Species by Automatic Partitioning) based on DNA barcoding of Cytochrome-C-Oxidase I (COI).Results: The molecular delimitation detected 72 to 81 Operational Taxonomic Units (OTU). Only five OTUs could be identified to species level by comparing sequences against the BOLD database using BLAST, and four more to the genus level. Adults and larvae could be successfully associated in 18 cases across six families. The high diversity of Trichoptera in this area highlights their potential as bioindicators for water quality assessment.Conclusions: This study provides an example of how molecular approaches can benefit the exploration of hidden diversity in unexplored areas and can be a valuable tool to link life stages. However, our study also highlights the need to improve DNA barcode reference libraries of Trichoptera for the Oriental region.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available