Morphology-Controlled Green Synthesis of Magnetic Nanoparticles Using Extracts of 'Hairy' Roots: Environmental Application and Toxicity Evaluation

Magnetic nanoparticles (MNPs) were green synthesized from a FeCl3/FeSO4/CoCl2 mixture using ethanolic extracts of Artemisia tilesii Ledeb 'hairy' roots. The effect of chemical composition and reducing power of ethanolic extracts on the morphology, size destribution and other features of obtained MNPs was evaluated. Depending on the extract properties, nanosized magnetic materials of spherical (8-11 nm), nanorod-like (15-24 nm) and cubic (14-24 nm) shapes were obtained via self-assembly. Microspherical MNPs composed of nanoclusters were observed when using extract of the control root line in the synthesis. Polyhedral magnetic nanoparticles with an average size of similar to 30 nm were formed using 'hairy' root ethanolic extract without any additive. Studied samples manifested excellent magnetic characteristics. Field-dependent magnetic measurements of most MNPs demonstrated a saturation magnetization of 42.0-72.9 emu/g with negligible coercivity (similar to 0.02-0.29 emu/g), indicating superparamagnetic behaviour only for solids with a magnetite phase. The synthesized MNPs were minimally aggregated and well-dispersed in aqueous medium, probably due to their stabilization by bioactive compounds in the initial extract. The nanoparticles were tested for magnetic solid-phase extraction of copper (Cu), cadmium (Cd) and arsenic (As) pollutants in aqueous solution, followed by ICP-OES analysis. The magnetic oxides, mainly magnetite, showed high adsorption capacity and effectively removed arsenic ions at pH 6.7. The maximum adsorption capacity was similar to 150 mg/g for As(III, V) on the selected MNPs with cubic morphology, which is higher than that of previously reported adsorbents. The best adsorption was achieved using Fe3O4-based nanomaterials with low crystallinity, non-spherical form and a large number of surface-localized organic molecules. The phytotoxicity of the obtained MNPs was estimated in vitro using lettuce and chicory as model plants. The obtained MNPs did not exhibit inhibitory activity. This work provides novel insights on the morphology of green synthesized magnetic nanoparticles that can be used for applications in adsorption technologies.

Automated summary

This study reported the green synthesis of magnetic nanoparticles using ethanolic extracts of Artemisia tilesii Ledeb 'hairy' roots. The chemical composition and reducing power of the extracts were found to influence the morphology, size distribution, and other features of the obtained nanoparticles. The synthesized nanoparticles demonstrated excellent magnetic characteristics and showed high adsorption capacity for copper, cadmium, and arsenic pollutants. The nanoparticles were also found to be minimally aggregated and well-dispersed in aqueous medium. Additionally, the nanoparticles did not exhibit inhibitory activity in vitro, indicating their potential use in adsorption technologies.