Journal
GENES
Volume 13, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/genes13122352
Keywords
Euonymus microcarpus (Oliv; ) Sprague; chloroplast genome; genetic diversity; phylogenetic analysis
Categories
Funding
- National Natural Science Foundation of China
- [32171701]
Ask authors/readers for more resources
We extracted genomic DNA from Euonymus microcarpus and generated its chloroplast genome using high-throughput sequencing and de novo assembly. The chloroplast genome contains multiple genes and repetitive sequences and provides a valuable reference for genetic diversity and evolution studies.
Euonymus microcarpus (Oliv.) Sprague, is a species of evergreen shrub of the genus Euonymus, family Celastraceae. Here, we extracted the genomic DNA from the leaves of E. microcarpus and constructed a paired-end library. The chloroplast genome of E. microcarpus was generated with the high-throughput sequencing by the illumina Hiseq X Ten platform and de novo assembly. The chloroplast genome had a quadripartite structure, containing a long single copy region with a size of 85,386 bp and a short single copy region with a size of 18,456 bp, separated by two inverted repeat regions of 26,850 bp. The chloroplast genome contained 133 genes identified in total, including 87 potential protein-coding genes, 38 transfer RNA genes, and eight ribosomal RNA genes. A total of 282 simple sequence repeats and 63 long repeats were found. Furthermore, the phylogenetic relationships inferred that E. microcarpus is sister to E. japonicus and E. schensianus. A comparison of the structure of the chloroplast genomes of eight Euonymus species suggests a nucleotide variability of the junction sites and a higher divergence of non-coding regions, compared to the coding regions. The original findings of the study serves as a good reference for chloroplast genome assembly and a valuable foundation for the genetic diversity and evolution of E. microcarpus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available