Journal
ADVANCED MATERIALS INTERFACES
Volume 10, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/admi.202201261
Keywords
CRISPR-Cas13a; finite difference time domain; gold nanomushroom; gold nanoparticle; localized surface plasmon resonance (LSPR); microfluidics; SARS-CoV-2
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A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) has been developed to achieve a significant plasmonic resonant shift. By optimizing the size, surface chemistry, and concentration of the AuNPs, the LSPR effect can be maximized. This system has been integrated with a CRISPR-Cas13a RNA detection assay for the detection of SARS-CoV-2 RNA targets, providing a simple, specific, isothermal, and label-free molecular diagnostic system.
A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite-difference time-domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR-Cas13a RNA detection assay for the detection of the SARS-CoV-2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label-free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.
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